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The making of this third edition is a revolutionary event in our life, as we went through a journey where we experienced how Microbiology can be changed to suit to the need of an Indian Medical Graduate. This book is prepared according to new MBBS curriculum and the content of the book was modified from the traditional organism-based teaching to system-based teaching. The content has been updated, concised and reshuffled—the three major types of changes incorporated in this edition. Parasitology has also been incorporated into this book under the respective infective syndromes, thus obviates the reading of a separate parasitology book. The reduction of the content is made by keeping the perspective of an Indian medical graduate in mind. The book is categorized into two parts, which is further divided into eleven sections.