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In the recent past there has occurred rapid changes in the syllabi of various universities. These syllabi have been opted from UGC syllabus. An attempt has been made to revise this book to incorporate these changes. All the techniques included in the earlier editions have been revised to incorporate the recent changes. At the same time some new techniques have been included. In each techniques, the different concepts have been simplified so that students may find the text more interesting. Every techniques is self sufficient and includes theory, instrumentation, applications, advantages and disadvantages. The order of techniques is still conventional and would be liked by students as well as teachers. This text expected to be useful for students of all streams, Analytical Chemistry, Organic Chemistry, Pharmacy, Bio-chemistry, Bio-technology, Bio-Inorganic Chemistry, Bio-Organic Chemistry, Environmental Chemistry, Medicine, Agriculture and Engineering Sciences. This text is also useful for anyone who uses chemical analysis in their work. Book Content of Analytical Chromatography 1. General Principles of Chromatography 2. Paper Chromatography 3. Thin-Layer Chromatography 4. Adsorption Chromatography 5. Liquid-Liquid Partition Chromatography 6. Liquid-Liquid Chromatography (LLC) on Bonded Phases 7. Liquid Chromatography-Mass Spectrometry 8. Countercurrent Chromatography 9. Gel Filtration or Gel (Exclusion) Chromatography or Exclusion (Permeation) Chromatography 10. Column Chromatography 11. Affinity Chromatography 12. High Performance (Pressure) Liquid Chromatography (HPLC) 13. Medium-Pressure Liquid Chromatography 14. Gas Chromatography 15. Gas-Chromatography Infrared Spectrometry 16. Gas-Chromatography Mass Spectrometry 17. Ion Exchange Chromatography 18. Electrophoretic Techniques 19. Capillary Electrophoresis 20. Capillary Zone Electrophoresis 21. Capillary Electro- Chromatography (CEO) 22. Micellar Electrokinetic Chromatography (MEKC or MEKC) 23. Ion Chromatography 24. Supercritic