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Biophysics is an interdisciplinary subject. This present text discusses the various aspects of Biophysics, giving approximately equal emphasis to these major aspects. It has been thus possible to show the inter-relationship of the different aspects of the subject in a coherent framework .This text has been written to be,as far as is possible, self contained, with most equations fully derived and critically discussed. It is supported by a large number of diagrams and micrographs which the author may feel necessary. Book Content of Biophysics Chapter 1: Introduction to Biophysics Chapter 2: Laws of Physics and Chemistry Chapter 3: Bio energetics Chapter 4: Carbohydrates Chapter 5: Proteins Chapter 6: Nucleic Acids Chapter 7: Lipids and Biological Membranes Chapter 8: Light Microscopy Chapter 9: Electron Microscopy Chapter 10: Radioactivity Chapter 11: Separation Techniques Chapter 12: Physico-Chemical Techniques to Study Bio molecules Chapter 13: Spectroscopy Chapter 14: Nuclear Magnetic Resonance Spectroscopy Chapter 15: X-Ray Crystallography Chapter 16: Model Building Chapter 17: Energy Pathways in Biology Chapter 18: Bio mechanics Chapter 19: Neurobiophysics Chapter 20: Origin and Evolution of Life