Immunological Techniques: Interpretations, Validation and Safety Measures

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Author: Ankita Joshi, Prof. R. S. Chauhan
Publisher: IP Innovative Publication Pvt. Ltd.
Edition: First
ISBN-13: 9789391208417
Publishing year: 2022
No of pages: 84
Weight: 150 g
Book binding: Paperback

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1. Ankita Joshi MSc, PDCR, ACPPM, MBA She is currently working as Contractual Scientist (PDF) in the Institute of Biotechnology (IBT), G.B. Pant University of Agriculture and Technology, Patwadangar, Nainital. She has done her graduation and masters in Biotechnology and also acquired diplomas and degrees in various other fields like PDCR, ACPPM and MBA. She is working on the development of anticancer therapy using traditional herbs present on Himalaya and Cowpathy. Her specialization is in animal cell culture techniques in which she has organized several Hand’s on training programmes for Young Biotechnologists. She has also prepared a laboratory manual on animal cell culture techniques besides several chapters in various manuals and books. One of her book is on Nanotechnology for beginners, which very popular amongst the Biotechnology students and researchers. She has contributed a lot in the field of Immunology and Immunopathology through research and development and organizing training programmes for young scientists. She had been awarded with Prakash Best Poster Presentation Award in National Symposium on ‘Immunobiotechnology’ held at IBT, G.B. Pant University of Agri. And Tech, Patwadangar in the year 2010. She is Life member and treasurer of the Society for Immunology and Immunopathology (SIIP). 2. R.S. Chauhan PhD, FNAVS, FSIIP, FIAVP, Diplomat ICVP, PDCR, ACPPM, OCTT, MBA During his tenure as academician and scientist, he has written 62 books including 21 manuals and 1 monograph; most of them are very popular among the students world over. He contributed 64 chapters in different books and published 185 research papers in various National and International journals of repute. Besides, he participated in 16 International and 57 National Conferences/Symposia/Seminars and presented 147 papers at different occasions. He also popularizes the scientific research among the common man by publishing 320 semitechnical articles in various magazines. He is life member of 15 Scientific Bodies/Societies/Associations and has been in several executive committee such as Chairman, PGPC; President, CTS; Secretary-General, SIIP; Vice President, ISVE; Zonal Secretary, IAVP; Joint Secretary, IVS; President, Dr. J.L. Vegad Foundation; Vice-President, IAVP; Registrar, ICVP, etc. Based on his contributions and scientific achievements, he has been awarded with several prizes, medals and honours including Best Young Scientist Award (1992), IAAVR Award (1996), National Fellow Award (1999), Fellow NAVS (2000), Fellow SIIP (2001), K.S. Nair Memorial Award (1999), Vigyan Bharti Award (2000), Dr. C.M. Singh Trust Award (2002), Dr. Rajendra Prasad Award (2002), Shri Ramlal Agrawal National Award (2000) Best Teacher Award (2004) by GB Pant University, Pantnagar, Fellow, IAVP (2006), Gopalgaurav, Bharat Excellence Award (2007), Diplomat, ICVP (2008), etc. in recognition of his research and teaching endeavor. He has been the principal investigator of 25 research projects worth millions of Rupees. The scientific contributions of Dr. Chauhan have been recognized internationally as Visiting Professor, University of Wageningen, The Netherlands and Temporary Advisor, WHO (Geneva). Dr. Chauhan ambitiously implemented quality management system (QMS) in CADRAD/CDDL and has got ISO 9001:2000 certificate for providing quality diagnostic services. He became Joint Director, CADRAD and Director of Indian Veterinary Research Institute and remained till completion of term in 2009. He also implemented uniform diagnostic methodology in all the Disease Diagnostic Laboratories throughout India. At present he is working as Director, Institute of Biotechnology, Nainital and pioneer in research on cowpathy and herbs for developing anti-cancer, anti-bacterial, anti-viral separations and universal immunomodulator without using synthetic chemicals.

<p>Immunity is the resistance of the body expressed through various cellular, physiological and biochemical reactions in the body. Whenever any foreign material/ infection enters in the body, the immune system activates and initiates production of immunity against those particular foreign material (infection/antigen). Mainly the immunity developed in the body is either in the form of humoral or cell mediated immune response and is specific to a particular antigen against which it is developed in the body. In the process of disease diagnosis, it is important to either detect the presence of antigen in the body or antibodies or specific T cells for the confirmation of the disease. For this purpose, various immunological techniques are employed to confirm the tentative diagnosis of physician/clinician. In the present book, various immunological techniques are described, including agglutination, precipitation, CFT, ELISA, DIA and other related techniques. These techniques are described in a very simple language and written by very experienced persons including the tit-bits of each tests so that the readers can understand easily and may employ these techniques in their research. Besides, validation 01 the techniques is described along with a diagnostic, sensitivity and specificity to determine the assay performance and reliability. Measurement of uncertainty related with each diagnostic test has also been discussed in this book. The safety measurement adopted in diagnostic laboratory along with the first aid in case of accidents are being well described. Each technique is well illustrated through diagram or photographs so that the basic principle of the technique is clearly understood. Authors arc having very rich experience of working in a diagnostic laboratory not only as technical expert or as a diagnostician, but also they are having the experience of research managers and management of immunological laboratory and diagnostic laboratory. 'Hope this book will be very useful to the young budding biotechnologists who are interested in immunological techniques, the teachers, the diagnosticians, the medicos and veterinarians in their studies, and research endeavours on the related topics. Authors are thankful to all those who helped in the preparation of this book, directly or indirectly. Lastly inspite of our best efforts at perfection element of human error is still likely to creep in which the readers are welcome to point out since that will help us in improving the book further.</p><div><br></div>