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This book is in resonance with the Textbook of Biochemistry for Medical Students, by Vasudevan, which is now in the 9th edition. It is prepared after consulting the revised MCI curriculum. The contents are divided into qualitative and quantitative experiments, which the students are supposed to do by themselves in the practical classes. In the end, a few case reports are also included, which will be useful for the students to prepare the practical examinations. Most of the chapters are rewritten so that this third edition is an upgraded version of the second edition.